Developments in the analysis of the regulation of chromatin structure have provided much information on their relevance in the control of DNA replication and transcription, making it an important area of research. Chromatin: A Practical Approach provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts. Both in vivo and in vitro methods are covered and clear explanations of procedures are provided throughout making this volume an invaluable aid to laboratory workers in biochemistry and molecular biology, especially those whose research encompasses eukaryotic transcription, DNA replication, and chromosome structure.